How is the measurement made?
The Thrombin Generation experiment is undertaken on a sample of plasma, which is commonly platelet poor. To this an initiator is added and the rate of thrombin generation is measured by the ability of the de novo thrombin to cleave a peptide from a fluorophore and induce fluorescence in the sample. The rate of development of the fluorescence is proportional to the thrombin present and is measured by the instrument to produce a time-trace known as a thrombogram. Watch the video below to see this in action!